Bruker Daltonics CLINPROT™ System is an integrated set of tools for biomarker discovery and clinical proteomics research. We offer established methodologies and techniques with powerful software packages to advance clinical research and shorten timelines in biomarker discovery.

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Watch the IMSC 2006 presentation "Clinical Biomarker Discovery Integrated with MALDI Molecular Imaging" in a 17 minute video.

One major objective is the discovery of potential biomarkers in complex body fluids like serum, plasma, urine, saliva or cerebral spinal fluid or tissue lysates or cell culture supernatants.

CLINPROT supports a profiling workflow to detect biomarker patterns indicative of specific diseases in biological fluids. In a second workflow individual biomarker candidates can be identified by means of the superior Bruker Daltonics TOF/TOF technology.

The CLINPROT toolbox

CLINPROT combines

Sample preparation

• innovative magnetic bead capture: CLINPROT magnetic beads
  CLINPROT magnetic beads are microparticles with dedicated, functional surfaces used to bind proteins and peptides. After elution the captured proteins and peptides are transferred to AnchorChips targets.
• Specialized sample support: AnchorChip^TM technology

Bruker Daltonics proven MALDI-TOF MS technology

• benchtop MALDI-TOF MS: microflex
• high-throughput: autoflex III, autoflex III TOF/TOF
• high-end MALDI-TOF MS: ultraflex III, ultraflex III

Profile spectra of the separated protein/peptide fractions are acquired using a well established FLEX-series MALDI-TOF instrument in linear or reflectron mode. The superior sensitivity and resolution of these instruments generate mass spectra with high information content. The AutoXecute™ acquisition control program enables automated operation with unsurpassed results.

ClinProTools software for biomarker detection and evaluation

Bioinformatics for all major functionalities in biomarker detection and evaluation:

• Data pre-treatment
• Powerful intuitive visualization of data from larger sample cohorts
• Peak statistics
• Pattern recognition with direct feedback to the visualization
• Crossvalidation of the cluster analysis and determination of sensitivity and specificity with independent test data
• Classification of unknown samples

BioTools software for biomarker ID

Any future use of biomarker candidates in clinical environments requires identification of the respective peptides. The TOF/TOF functionality offered by CLINPROT addresses this important task in clinical proteomics. Biomarker candidates detected by ClinProTools can be subjected to TOF/TOF analysis. Metastable fragment ions of the respective precursor ion are analyzed after a second acceleration step. The BioTools software package interprets the resulting fragment pattern and is used for peptide identification via database search.

The CLINPROT micro is a new affordable, easy-to-use and compact clinical system dedicated to peptide and protein profiling, biomarker discovery and biomarker validation. It is based on a high-performance bench-top MALDI-TOF mass spectrometer from Bruker Daltonics’ highly regarded FLEX series, providing excellent biomarker detection sensitivity and resolution, as well as the reliability and reproducibility needed for rigorous validation studies of putative biomarkers for cancer and other diseas

CLINPROT workflow

The CLINPROT micro system offers unique integrated sample preparation tools for biological fluids and tissue extracts with comprehensive analysis, visualization and model building software. The well-established CLINPROT workflow easily measures peptides and proteins which can be used to discover multi-marker panels or profile patterns indicative of specific diseases. These multi-marker panels or patterns have potentially better diagnostic specificity than single biomarkers.

The solution package

The ClinProt Micro solution is ideal for cancer research and other clinical and diagnostics research laboratories engaged in biomarker discovery across a range of samples.

• unique, CLINPROT magnetic bead kits for facile, scaleable and highly reproducible sample preparation
• robust and easy-to-use MALDI-TOF system
• integrated Compass™ software for intuitive operation by expert and non-expert users alike
• patented AnchorChip™ MALDI target technology for fast automated acquisition and a 10–100 fold sensitivity increase
• ClinProTools bioinformatics system for smart visualization of large numbers of profiles, the creation of predictive models, the validation of models via cluster analysis, and the classification of unknown samples - addressing all the major functions in biomarker pattern profiling and validation
• optional upgradeable with reflectron technology for biomarker identification and detection of post-translational modifications.

For research use only. Not for use for diagnostic purposes.