Challenges in the investigation of the metabolic changes in Nicotiana attenuataduring insect herbivory using an improved HPLC-TOF-MS method

Poster

Metabolomics Studies

DGMS 2010

OTOF

2010

micrOTOF, micrOTOF-Q, ProfileAnalysis

Matthias Schöttner (1), G. Zurek (2), E. Rothe (1), E. Gaquerel (1), B. Berger (1), V. Tellström (2), B. Schneider (2), A. Barsch (2), Ian T. Baldwin (1); (1) Max Planck Institute for Chemical Ecology 07745 Jena – Germany (2) Bruker Daltonik GmbH, 28359 Bremen – Germany

DGMS

Abstract

• By means of internal standards and a pooled quality control sample, the reproducibility of the analytical method was monitored.
• PCA and t-test were successfully applied to filter out metabolic changes.
• The identification of regulated metabolites can be achieved based on molecular formulae derived from accurate mass spectra plus chemical knowledge.
• This method will be applied to investigate the influence of the genetic modification.

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DGMS 2010

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