Protein profiling and identification in complex biological samples using LC-MALDI
| Type: | Application |
Journal Article |
Expression Proteomics |
| Number: | Technology |
DP0905 |
MALDI-TOF |
| Year | Products |
2005 |
PROTEINEER-LC, PAC, ultraflex, autoflex, CLINPROT magnetic beads |
| Author | |
Dr S. Brand, Dr S Hahner and Dr R Ketterlinus |
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| Reference | |
DrugPlus international - September 2005 |
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Abstract |
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Offline-coupling of liquid chromatography (LC) to MALDI-mass spectrometry (LCMALDI) is an method for the analysis of complex biological samples that is becoming more and more widely used. The increasing popularity of this technique is due to several reasons. Firstly, the approach is relatively straightforward and secondly, once fractions are spotted on MALDI-targets there are no time constraints on carrying out further analyses. This means that intelligent data-dependent MS/MS analysis can be carried out on the basis of prior MS and MS/MS analysis, resulting in significantly greater protein identification success rates. This article describes the LC-MALDI technique and illustrates its power through an example analysis involving the complex lysate of Corynebacterium glutamicum ATCC13032. 350 non-redundant proteins were clearly identified in the lysate. |
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