Challenges in the investigation of the metabolic changes in Nicotiana attenuata during insect herbivory using an improved HPLC-TOF-MS method
| Type: | Application |
Poster |
Metabolomics Studies |
| Number: | Technology |
WPD-120 |
UHR-TOF |
| Year | Products |
2009 |
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| Author | |
Matthias Schöttner1; Eva Rothe1; Emmanuel Gaquerel1; Beatrice Berger1; Birgit Schneider2; Gabriela Zurek2; Aiko Barsch2; Mike McDonnell3; Ian T. Baldwin1 |
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| Reference | |
Abstract |
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• Reserpine and atropine served as standards for quality control (RSD of peak areas < 10%). A pooled QC sample was applied as ‘chemical average’ to monitor real sample behaviour. • PCA of all WT samples clearly identified trends for metabolic changes at late time points (86 &120h). • T-test was successfully applied to determine regulation ratios in 120h WTcontrol vs WT+OS. • Based on accurate MS/MS data and chemical knowledge structural hypothesis can be generated, as shown for 484.2442m/z. • The same procedure will be applied to investigate the influence of the genetic modification. |
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