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HPLC-TOF-MS method HPLC-ESI-TOF-MS and statistical analysis of metabolic changes in Nicotiana attenuatainduced by insect herbivory

Type: Application

Poster

Expression Proteomics, Gen. Pharma & Chemistry
Number: Technology

PMM-409

UHR-TOF, OTOF
Year Products

2009

  Author
 

Matthias Schöttner1, Beatrice Berger1, Emmanuel Gaquerel1, Eva Rothe1, Birgit Schneider2, Aiko Barsch2, Gabriela Zurek2, Ian T. Baldwin1
  Reference
 

Poster IMSC 2009
 

Abstract
 

  • By means of internal standards and a pooled QC sample, the reproducibility of the analytical method was monitored.
  • PCA and t-test were successfully applied to filter out metabolic changes.
  • The identification of regulated metabolites can be achieved based on molecular formulae derived from accurate mass spectra plus chemical knowledge.
  • This method will be applied to investigate the influence of the genetic modification.
 

Related Products
 

IMSC 2009

 

Download

 

IMSC2009-GZ-PMM409.pdf
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