Improved method for fungal identification using MALDI-TOF mass spectrometry
| Type: | Application |
Poster |
Clinical Proteomics |
| Number: | Technology |
DMykG - 2009, P22 |
MALDI-TOF |
| Year | Products |
MALDI Biotyper |
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| Author | |
Thomas Maier & Markus Kostrzewa Bruker Daltonik GmbH Bremen Germany |
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| Reference | |
Abstract |
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MALDI-TOF mass spectrometry fingerprint analysis has emerged as a valuable tool for microorganism identification and classification during the recent years. While for bacteria and yeasts several studies have reported simple and robust approaches for sample preparation, leading to reproducible results with low impact of cultivation conditions and growth state, the situation for filamentous fungi keeps more complicated. In particular, sample preparation of molds with rigid cell walls is more challenging and the fingerprint spectra derived from spores (and spore containing material) and mycelium show significant differences. Further, fungi grown on solid media frequently show strong adherence with the agar and harvesting without medium hardly can be achieved. Additionally, spore forming regions or regions initiating germination cannot be separated for harvesting. Further, growth media obviously have a considerable effect on mass spectra. Here, we demonstrate a novel approach for analysis of filamentous fungi by MALDI-TOF MS, based on liquid culturing improving reproducibility and quality of spectra, significantly. |
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