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The Role of Increased Resolution and Scan Speed of Ion Traps for Top Down Proteomics with ETD/PTR
| Type: | Application |
Poster |
Expression Proteomics |
| Number: | Technology |
ThPH-207 |
Ion Trap |
| Year | Products |
2009 |
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| Author | |
Michael Schubert, Christian Albers, Andreas Brekenfeld, Christoph Gebhardt, Eckhard Schwabe and Ralf Hartmer |
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| Reference | |
Abstract |
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•amaZon´s maximum resolution scan mode (4600 u/sec) allows the seamless and fast detection of a plethora of multiply charged ETD-fragments (z = 1 – 6) •Data processing with SNAP II generates a list of monoisotopic ETD-fragment masses, enabling the ID of the intact protein by a Mascot data base search •In depth top-down sequence characterization of proteins up to MW of 20 kDa |
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