Utilization of high-accuracy FTICR-MS data in protein quanitation experiments
| Type: | Application |
Poster |
Expression Proteomics |
| Number: | Technology |
FTMS |
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| Year | Products |
2009 |
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| Author | |
Martin Strohalm1,2, Petr Novák1, Petr Pompach1, Petr Man1, Daniel Kavan1, Matthias Witt3, Pert Džubák4, Marián Hajdúch4, Vladimír Havlíček1,4 |
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| Reference | |
Abstract |
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By using peptide mass fingerprinting with 1.5 ppm mass tolerance we were able to utilize farmore data retained in MS scans which would normally be missed by a standard processingmethod. We have significantly increased the number of peptides used for protein quantitationand also the sequence coverage. Altogether, it enabled us to perform a more accuratequantitation as well as to search and identify some interesting post-translational modifications.In addition, we used this technique to distinguish among closely related protein isoforms,commonly returned by Mascot search engine. Nonetheless, it has to be mentioned, that evenwith the high mass precision and accuracy of FTICR-MS instrument, false positive matches canalso appear as the sample complexity increases. If the extremely powerful combination ofonline LC-ESI-FTICR-MS and offline LC-MALDI-TOF-TOF-MS is applied, however, this newapproach of data processing can be successfully used in candidate selection process forsubsequent offline detailed mass analysis. Visit |
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