Expression Proteomics
MALDI- and ESI-MS/MS are the core technologies for protein identification and in-depth characterization in expression proteomics. Adjusted automation suites are essential for successful sample tracking, archiving and retrieval for low to high throughput approaches.
Watch the IMSC 2006 presentation "ETD and beyond" in a 14 minute video.
Analytical challenge | Methods | Our Solution | Further Reading |
High-success Protein Identification and Characterization (2D Gel based) | Gel Imaging and Spot Picking | ||
Protein Digest and MALDI Preparation | |||
MALDI-TOF Peptide Mass Fingerprinting | |||
MS/MS Peptide Fragment Analysis | |||
Characterization of PTMs | |||
De novo Sequencing | |||
Prespotted AnchorChip | |||
smartbeam | |||
High-success Protein Identification and Characterization (LC-based) | LC-MALDI-MS/MS | ||
LC-ESI-MALDI-MS/MS | |||
2D-LC-MS/MS | |||
Prespotted AnchorChip | |||
smartbeam | |||
Top-Down Sequencing of Intact Proteins | T3 Sequencing | ||
Top>Pro (ECD-Qq-FTMS) | |||
smartbeam | |||
Protein Identification from organisms without sequenced genomes | De Novo Sequencing | ||
Protein Quantitation | Stable Isotope Labeling | ||
MALDI Imaging | ImagePrep sample preparation | ||
Analysis of Intact Proteins and Protein Complexes | ESI-TOF MS | ||
Analysis of Noncovalent Protein/Ligand Complexes | ESI-TOF MS | ||
PTM Discovery | ETD and CID | ||
Erythropoietin Characterization / Glycoproteine Characterization | CE-MS |
TN 003