The comprehensive analysis of proteins down to the level of their primary amino acid sequence and monitoring of any post-translational modifications are analyses that are not readily accessible utilizing traditional enzymatic digest based MS methods linked with a subsequent database search. At best, the traditional, multi-step, labor intensive approach to protein sequence analysis provides 20-80% sequence coverage and does little to measure post translational modifications. While useful for some studies, this technique is often not sufficient for the complete characterization of biopharmaceuticals where point mutations, splice variants, and sequence deletions, etc. can have a significant affect on the efficacy or pharmacology of a biotherapeutic.

Read this article to learn how Top-down sequencing of proteins dramatically increases the level of information to provide a more complete picture of protein sequence and structure.

The primary structure of a 13.6 kDa single heavy chain camelid antibody was determined by matrix-assisted laser desorption ionization-time-of-flight/time-of-flight (MALDI-TOF/TOF) top-down sequence analysis.

To our knowledge, this is the first time that the entire primary structure of a protein with a molecular weight >13 kDa has been established by mass spectrometric top-down sequencing.

Read more: Top-Down de Novo Protein Sequencing of a 13.6 kDa Camelid Single Heavy Chain Antibody by Matrix-Assisted Laser Desorption Ionization-Time-of-Flight/Time-of-Flight Mass Spectrometry   

Learn more about Top-Down Protein Sequencing

Determining the outcome and analyzing the results of a chemical reaction is a key step in many chemistry groups. Performing these assays requires an easy to use, sensitive, robust LCMS system capable of performing routine analyses at a variety of throughput levels.  The amaZon SL is the newest member of a long line of Ion Trap Mass Spectrometers that with its combination of sensitivity, speed, and performance makes it an ideal component of an LCMS system for monitoring the results of chemical reactions.

Learn more: Technical Note TN-38. Fast and reliable Reaction Yield and Compound Purity Assessment in Synthesis Quality Control 

ProfileAnalysis 2.0 available

Make the most out of your Metabolite Profiling and Label-free quantitation workflows: New features like retention time alignment, t-test calculation and numerous options for data scaling and normalization have been implemented in the new version of ProfileAnalysis 2.0. These options complement the well established functions such as PCA and SmartFormula^TM. 

Download at:  Downloads & Patches 

DataAnalysis 4.0 SP 2 available with CompoundCrawler 1.0.

CompoundCrawler adds a further dimension to compound identification: Results from SmartFormula can easily be submitted to various compound databases. After import of the results of database searches, comprehensive information is at your fingertips for further evaluation including the visualization of compound structures.

Download at:  Downloads & Patches