A 9.4 T Fourier transform ion cyclotron resonance mass spectrometer: description and performance
| Type: | Application |
Scientific Paper |
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| Number: | Technology |
L00:2:739 |
FTMS |
| Year | Products |
2000 |
Apex |
| Author | |
Palmblad, M.(1), Hakansson, K.(1), Hakansson, P.(1), Feng, X.(2), Cooper, H.J.(2), Giannakopulos, A.E.(2), Green, P.S.(2), Derrick, P.J.(2) |
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| Reference | |
Eur. J. Mass Spectrom. 6(3) 2000 267-275 |
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Abstract |
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Commercial FT/ICR mass spectrometers installed in both labs. are described and their performances illustrated by the anal. of peptides, proteins, oligonucleotides, fullerenes and synthetic polymers. The instrument features easily interchangeable ion sources: MALDI, SIMS, EI/CI and electrospray (ESP). MS: Bruker Daltonics BioApex 94e, FT/ICR, 9.4-T, ESP: Analytica source, N2 nebulizing gas, heated drying gas, or lab.-built ESP: 0.1 mm i.d. stainless steel needle etched to conical shape, no nebulizing or drying gas, shutter to permit multipole storage-assisted dissociation, MALDI: N2 laser, 337 nm. The lower mass limit is m/z 29 and the highest obsd. single charged m/z value is 8565 (by MALDI). Resolution is > 4,000,000 for m/z 130.9916 from PFTBA and is > 1,200,000 for ubiquitin ([M+10H]10+) by ESP. There is clear resolution between C59N+ and C58 13C2 + (mass diff. 3.6 mDa). |
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