New CLINPROT magnetic beads
The new Glyco Kit MB-LAC WGA (Wheat Germ Agglutinin) complements the glyco bead product line. While the existing Glyco Kits MB-LAC ConA and MB-CovAC Boronic are suitable for capturing of glycosylated proteins and peptides in a more general manner, the MB-LAC WGA captures respective molecules with a more specific binding motif:
The receptor sugar for WGA is N-acetylglucosamine preferentially binding to dimers and trimers of this sugar. WGA can also bind oligosaccharides containing terminal sialic acids or chitobiose.
ConA (Concanavalin A) binds to most N-glycosylated proteins, due to the conserved penta-core structure of glycoproteins containing mannosyl and glucosyl residues with free hydroxyl groups at positions C3, C4 and C6.
Boronic acid forms covalent bonds to molecules containing cis-diol groups, like mannose, galactose or glucose and captures therefore in addition to N-glycosylated also O-glycosylated and C-glycosylated proteins carrying respective groups.
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Bruker Daltonics Glyco Kits allow direct capturing of glycosylated peptides and proteins from human serum or other complex protein sources and the subsequent characterization as well as identification by MALDI-TOF and MALDI TOF/TOF analysis.
Combining the respective specific glyco-capturing beads, tryptic digest and MB-HIC C8 purification facilitates identification of serum glycoproteins contributing to the fraction making up 1% of serum proteins. Each bead type presents its own individual binding profile that partially also overlaps with the profiles of the two others functionalities.
Available CLINPROT magnetic beads kits
Profiling Kits | ClinProt Starter Kit, MB-HIC 8, MB-WCX, MB-WAX, MB-IMAC Cu |
Large-Protein Beads | MB-HIC 1, MB-HIC 3 |
Peptide Beads | MB-HIC 18 |
Phospho Beads | MB-IMAC Fe |
Glyco Beads | MB-LAC Con A, MB-CovAC boronic, MB-LAC WGA |
Antibody Capture Beads | MB-IAC Prot G |
