The SERVA ICPL-Kit
for Quantitative Proteomics
A new dimension in the quantitative MS-analysis of proteomes
- New labeling technology for protein quantitation by mass spectrometry.
- Labeling on protein and peptide level possible.
- Compatible with protein fractionation for high proteome coverage and high sensitivity.
- Compatible with isoform and modified protein detection.
- Automated analysis and robust quantitation statistics.
- For all mass spectrometers including the HCT™ Ion Traps.
Fully implemented in the PROTEINEER-LC™ 1.1 suite.
Labeling prior to fractionation
Successful proteomics projects require protein fractionation resulting in a higher sensitivity and proteome coverage.
The new ICPL™ technology (ICPL = Isotope Coded Protein Labeling) supports this promising strategy since it allows protein labelling before fraction and thus maintains the quantitative ratios.
Workflow
- Cell lysis and ICPL labelling of the different cell states.
- Combination of both samples.
- Optional reduction of complexity on protein level, e.g. by 2D GE, PAGE, LC or CLINPROT magnetic beads.
- Hyphenation of fractionation strategies is possible.
- Enzmatic digestion of proteins.
- Optional reduction of complexity on peptide level by LC.
- Mass spectrometry (Quantitation, Identification) and automated statistical analysis in PROTEINEER-LC.
The SERVA ICPL-Kit was
invented by Schmidt, Lottspeich & Kellermann (
Proteomics 2005, 1, 4–15),
developed by TopLab, Munich,
is produced by SERVA Electrophoresis Heidelberg
and available through 
Bruker Daltonics CARE online.
Further Readings
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