micrOTOF II

The powerful benchtop ESI-TOF MS - enjoy the new flexibility

The micrOTOF™ II provides powerful exact mass measurements in an easy-to-use benchtop package. Featuring excellent mass resolution and mass accuracy in combination with true isotopic pattern, micrOTOF II is the perfect choice for straight-forward formula determination of small molecules, quality control of intact proteins, and metabolic studies.

Watch the IMSC 2006 presentation "Integrated Metabolic Profiling" in a 15 minute video.

Superior mass accuracy

Superior mass performance characteristics are achieved by combining Bruker Daltonics’ successful time-of-flight (TOF) technology with innovative detection technologies. The micrOTOF shows a mass accuracy of better than 2 ppm without sacrificing resolution or sensitivity. A mass accuracy of better than 5 ppm can be achieved with external calibration without any suppression effects of calibrants and while maintaining the high duty cycle.

TIP™ – True Isotopic Pattern with SmartFormula algorithm

Replacing the former approach of just using the measured mass for formula determination, the micrOTOF II adds a second dimension into the routine: Analysis of the isotope profile provides the so-called “True Isotopic Pattern” (TIP) which is matched against the measured spectrum – leading to results with significantly increased confidence.

Unsurpassed resolution

Featuring a mass resolution exceeding 10,000 FWHM (exceeding 16,500 with micrOTOF II focus), the micrOTOF II defines the new standard in ESI-TOF mass spectrometry. True isotopic resolution can be observed even for deconvoluted spectra at high resolution. The simulated pattern fits perfectly to the measured results.

Wide range mass accuracy

The micrOTOF II knows how to impress with an excellent stability of the mass measurement over a wide dynamic range without tedious recalibration routines:

An advanced temperature stabilization keeps the mass position stable for hours without the need of time-consuming recalibration procedures.
The mass accuracy with external calibration stays stable even with significantly variations in sample concentration.

High flexibility by new APCI source

By a new developed APCI source the micrOTOF II can be used in combination with an LC or a GC likewise. The system can be switched from LC to GC and vice versa within minutes, thus providing the user with the performance of a real high-resolution TOF-MS for his GC problems. The micrOTOF II is the only oTOF-MS worldwide which can be used in combination with LC and GC!

The source can easily be upgraded to APLI, thus demonstrating outstanding performance in sensitivity and selectivity for applications like PAH analysis.

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Powerful software environment

The Compass software environment handles all aspects of system control and data handling with an intuitive and easy-to-use graphical user interface (GUI). The LC/MS workflow can be fully automated with a simple method approach. Compass integrates the micrOTOF II with all popular HPLC and nanoLC systems to match your separation needs.

The micrOTOF II system can be adapted to an “Open Access Environment” covering typical aspects of multiple users with various samples.

Applications

The formula machine

With the micrOTOF II, straight-forward formula determination and confirmation is only a mouse-click away. The GenerateFormula tool lists and rates possible formulas consistent with the mass measurement. Furthermore, the true isotopic pattern (TIP) is used for significantly increased specificity: If the given mass accuracy leads to multiple possible answers, the TIP adds a second dimension to the analysis, since most wrong hits can be excluded due to differences in the isotopic patterns.

Metabolic Profiler

The micrOTOF II is the core of the Metabolic Profiler solution for metabolism studies and analysis of complex mixtures.

Analysis of intact proteins and other large molecules

The sensitive detection and accurate mass determination of intact proteins is a challenge readily answered by the micrOTOF II. Isotopically resolved spectra are obtained up to a mass of about 15 kDa, and modifications and complex formation can be investigated.

Fast LC/MS and CE/MS

Solve your analytical problem by combining speed with high resolution and mass accuracy: Fast data acquisition and our grounded needle concept give easy access to fast chromatographic and CE applications. Full real-time processing includes peak picking, profile-to-line spectra conversion, automatic recalibration and data reduction.

Technical Data

Sophisticated micrOTOF II technology

World-leading combination of mass accuracy, resolution, and sensitivity
Unrivalled flexibility by widest variety of sources
Detection of ions with True Isotopic Pattern (TIP™) and SmartFormula™
Automated determination of elemental composition, the “formula finder”
Footprint 640 x 640 mm, height 1220 mm, weight 130 kg

TOF analyzer

Standard mass range 50 – 3,000 m/z
Extended mass range 50 – 20,000 m/z
Mass resolution >10,000 FWHM in standard configuration and >16,500 FWHM with micrOTOF II focus
Mass accuracy of 2 ppm (RMS) error with internal calibration
Mass accuracy of 5 ppm (RMS) error with external calibration
Acquisition rate of 20 Hz (full mass range profile mass spectra to hard disk)
Polarity switching for both, positive and negative ions

Source options

ESI orthogonal electrospray source (1µl –1 ml/min)
APCI orthogonal atmospheric pressure chemical ionization source (100µl –1,5 ml/min)
APPI Atmospheric pressure photo ionization source
Online NanoElectrospray source for capillary LC applications, flow down to 50 nL/min
Offline NanoElectrospray source, consumption of 1–2 µL per sample @ 25 nL/min
CE-MS coupling with grounded ESI needle
GC-APCI source for coupling with GC and LC