autoflex III & autoflex III TOF/TOF

High-Performance MALDI-TOF & TOF/TOF Systems

Innovative MALDI-TOF and TOF/TOF technology for reliable and detailed protein characterization, high resolution MALDI imaging and validated biomarker discovery. Patented smartbeam™ laser technology is a must for MALDI Molecular Imaging and gives access to superior performance in any Proteomics project.

Watch the IMSC 2006 presentation "Clinical Biomarker Discovery Integrated with MALDI Molecular Imaging" in a 17 minute video.

Just one click to expert results

Succeeding the well-established autoflex II system, the autoflex III incorporates in addition our proprietary PAN™ (panoramic) broadband mass focusing. It allows impressive simultaneous mass resolution across a wide mass range leading to optimum results without tuning or changing parameters. An on-site upgrade to full TOF/TOF capability is possible.

Intuitive instrument control

The autoflex III operates within the powerful Compass software environment. Compass offers a seamless link with the most comprehensive bioinformatics software suite in the industry. Dedicated software solutions e.g. flexImaging™ for MALDI Molecular Imaging of tissue samples or ClinProTools™ for biomarker discovery and biomarker validation are available.
The only solution package to offer direct Class Imaging™ of clinical samples.

Consistent automation

The microtiter plate format of the MALDI sample target guarantees full compatibility with the biochemical lab and is essential for high-throughput sample preparation (e.g. on PROTEINEER dp robot). The powerful proprietary AnchorChip™ and Prespotted AnchorChip target technology provides homogeneous, exactly-positioned samples on the MALDI target for robust and rapid automated data collection, as well as a sensitivity boost by up to two orders of magnitude.

Reliable long-term operation

All system components have been designed and tested for maximum life and minimum wear. The service concept includes automated self-diagnosis routine and remote service capabilities.

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Applications

Advanced Biomarker Discovery
The autoflex III is the system of choice for high-resolution peptide and protein biomarker discovery, identification and validation. The integrated CLINPROT solution provides automatic magnetic bead based sample preparation and software tools for data analysis, visualization and statistical model building.

MALDI Imaging

Best choice for MALDI Molecular Imaging applications. Tissue samples can now be scanned with an adjustable high repetition rate from 1 to 200 Hz, providing maximum sensitivity. The integration of easy-to-use ClinProTools™ and flexImaging™ bioinformatics solutions creates a powerful combination for peak profile classification and MALDI imaging in investigating the distributions of peptides and proteins in tissue samples, known as Class Imaging™ (Patent pending).

High-success Protein Identification

The autoflex III TOF/TOF enables ultra sensitive protein identification by MALDI-TOF peptide mass fingerprinting, immediately followed by more detailed protein characterization using MALDI-TOF/TOF tandem mass spectrometry (MS/MS) on the same prepared sample. The PROTEINEER and PROTEINEER-LC system solutions provide efficient workflows for both 2D gel based and LC based approaches.

Functional Genomics

The autoflex III is an integral part of the GENOLINK solution for functional genomics. In a combined PROTEINEER/GENOLINK environment, the autoflex III supports combined genetics, gene expression and proteomics approaches and thus opens new ways of discovering disease markers and drug targets.

Technical Data

MALDI source

scoutMTP™ Ion Source with state-of-the-art PAN™ panoramic mass range focusing
smartbeam™ laser technology for uncompromised performance based on an All-Solid-State Laser system

Automation

Patented AnchorChip™ MALDI target technology for fast automation, increased sequence coverage and 10–100 fold sensitivity increase over stainless steel target holder
MALDI targets in 384 and 1536 microtiter plate format
Target identification via transponder or optional barcode reader
Compatible with Bruker Daltonics’ sample preparation robotics

autoflex III TOF Analyzer

Dramtically enhanced resolution, mass accuracy and sensitivity
High resolution reflectron configuration available
Optional MS/MS capability with autoPSD (automated post-source decay) including pre-cursor ion selection device
Modular design allows for future upgrades to TOF/TOF

autoflex III TOF/TOF Analyzer

Latest TOF/TOF technology (LIFT™) enables the use of various MS/MS techniques (e.g. LID, high energy CID) for fast and sensitive MS/MS experiments
Patented gridless ion source and reflector design

Compass™ software suite

flexControl™ module for quick and easy instrument control
Fuzzy-logic based AutoXecute™ engine for on-the-fly optimization of spectral quality
flexAnalysis module for automated and interactive data analysis, including integration with BioTools™, ProteinScape™, ClinProTools™, GenoTools™, and search engines
Optional LC-MALDI (WARP-LC™) and MALDI Imaging (flexImaging) software solution
Optional Compass Security Pack™ for work in regulated environments (supports 21CFR part 11 compliance)