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EASY-nLC

Tailored HPLC for nano-LC-MS Proteomics

"One Click" separation of components

It is easy to handle, steady and reliable. A 1D-nanoflow HPLC system tailored to the requirements of today’s proteomics applications. The EASY-nLC provides the researcher with:


  • Simplicity: Easy installation & operation
  • Reliability: Robust & steady
  • Low-Maintenance: Split-free gradient mixing
  • Efficiency: minimum peak widths < 5 sec.
  • Compact Design with small footprint

Ease Of Use and Split-Free Flows

The EASY-nLC is a compact, innovative and affordable nano-HPLC system for state-of-the-art proteomics laboratories. Split-free binary gradient mixing down to the low nanolitre/min range are made possible by precise direct drive pumps. Bruker Daltonics‘ CompassTM software environment fully integrates the EASY-nLC with Bruker Daltonics mass spectrometry systems.

Easy installation and handling

The system is easily controlled by the intuitive and familiar Bruker CompassTM HyStarTM software from hardware setup to method definition, sample definition and data acquisition. While standard HPLC operations, like solvent purging, air removal and column flushing are performed automatically, first analytical data are easily available 4 hours after power-up.

Split free liquid flow system

Precise nanolitre pumps allow very exact split free binary gradient mixing. Advanced Flow Control (AFCTM) ensures accuracy and reproducibility with two nanoflow sensors – one for each mobile phase – and also allows individual and automatic calibration of the two solvents.

Sample overlay demonstrates excellent reproducibility
Excellent chromatographic performance is demonstrated by 4 seconds peak widths in high resolution extracted ion chromatograms (hrEIC).

Technical Specifications

Performance

  • Split-free flow down to 20 nL/min
  • Pulse-free flow
  • Innovative design for 1D separations with and without trapping column
  • AFC allows automatic calibration of solvents
  • Highly reproducible chromatograms
  • Chromatographic performance with peak widths around 3 - 5 seconds
  • No online degassing required
  • Pre-defined routines for purging and bubble removal

Layout

  • Cooled autosampler (down to 4 °C)
  • Accommodates 24 sample vials or 1 microtiter plate with 96 or 384 wells.

Compass software suite

  • Integration in Compass Software Interface for hardware setup, method definition, sample setup, and data acquisition.
  • Optional Compass Security Pack for work in regulated environments (21CFR part 11 compliance)

Service features

  • Remote on-line service capability via Internet sessions
  • Extended self diagnostics
  • Supplementary IQ/OQ/PV procedures available

For research use only. Not for use for diagnostic purposes.

EASY-nLC is a trademark of P Proxeon A/S, Odense, Denmark

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Further Readings

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