PROTEINEER-LC

LC-MS/MS-based Proteomics Suite

Watch the IMSC 2006 presentation "ETD and beyond" in a 14 minute video.

For more quality data and more knowledge in LC-MALDI MS/MS, LC-ESI-MALDI MS/MS, 2D-LC-MS/MS and Quantitative Proteomics.

LC-MS/MS based analytical strategies are rapidly moving into focus for elucidating complex proteomics samples. They are used as complement to the classical gel-based approach. Mass spectrometric information is generated with online-ESI- and/or offline-MALDI-MS/MS, both technologies delivering complementary protein sequence data for maximum information depth.

Bruker Daltonics offers a comprehensive, integrated platform to support all major LC-based proteomics strategies: PROTEINEER-LC™!

The PROTEINEER-LC toolbox

Sample Preparation and LC Separation

All widely used CapLC and nanoLC systems supported by PROTEINEER fc™ MALDI Fraction Collector

Mass Spectrometry with

ultraflex III™ TOF/TOF
autoflex III™ TOF/TOF
HCT™ series
ESI-Ion Trap MS systems

Data Interpretation with

WARP-LC Protein Browser
LC Run Survey

Knowledge Generation with

ProteinScape™ Database System for Proteomics Project Management

Workflow Control

The WARP-LC™ software module supports various LC-based proteomics workflows and controls data acquisition throughout the analytical process. The intelligent precursor ion selection for MALDI MS/MS takes isotopic labelling or LC-ESI results into account. This strategy provides maximum information content while minimizing sample consumption and analysis time. Samples are tracked at any stage by Bruker Daltonics’ unique transponder technology.

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Technical Specifications:

Target Applications

High-success protein identification from complex protein mixtures, starting with 1D- or 2D-LC separation
Detailed characterization of PTMs, polymorphisms, etc.
Quantitation by stable isotopic labeling experiments, such as ICAT™*
LC-ESI-MS/MS, LC-MALDI-MS/MS, and combined LC-ESI-MALDI strategies supported

LC and Sample Preparation

All widely used nanoLC and Capillary LC systems fully supported
PROTEINEER fc™ Fraction Collector for accurate preparation of AnchorChip™ MALDI targets (LC-MALDI- and LC-ESI-MALDI workflows)

Mass Spectrometry

High-performance ESI-ion trap MS/MS and MALDI-TOF/TOF technology from one vendor:

Ultra-fast LC-ESI-MS/MS with highest sensitivity and dynamic range
Advanced MALDI-TOF/TOF MS with AnchorChip technology for higher sequence coverages, search scores, and success rates of protein ID
MALDI targets in MTP format (384 and 1536)
Prespotted AnchorChip™ - disposable MALDI targets for elimination of cross contaminations and storage for re-analysis

Workflow Management

Complete software integration of all system components, including full sample tracking through transponder reading
WARP-LC™ software for LC-based proteomics workflow control
Intelligent result feedback for maximum sample readout at minimum time

Data Interpretation and Knowledge Generation

Comprehensive set of tools for validation and reporting of results:

LC Run Survey™ for quick evaluation of entire LC-MS/MS runs
WARP-LC Protein Browser to report identified proteins and peptides
BioTools™ software for automated and interactive interpretation of MS and MS/MS data, providing analysis tools for mutations, polymorphisms, and modifications, such as de novo sequencing
ProteinScape™ database solution for proteomics project management

For research use only. Not for use for diagnostic purposes.

*ICAT is a trademark of the University of Washington.