PROTEINEER

Mass Spectrometry-Based Proteomics Suite

The PROTEINEER™ suite integrates our powerful mass spectrometers with robotics and bioinformatics to deliver maximum productivity and success in proteomics projects.

PROTEINEER is designed for ultimate sensitivity, success rate, and throughput of protein identification.

High performance of all system components, combined with a unique automated and intelligent workflow control (WARP™), provide an unmatched overall productivity, enabling "High Success Proteomics" at a minimum of running costs and effort. PROTEINEER is scalable and can be configured for use in small pilot phase studies up to full-blown industrial-scale proteome initiatives with thousands of samples per day.

The PROTEINEER toolbox

Sample preparation from 2D gels:

• Automated gel imaging, spot picking, digestion and MALDI preparation on the PROTEINEER spII and PROTEINEER dp robotic platforms

Mass Spectrometry:

• our leading automated MALDI-TOF and TOF/TOF systems for high-success peptide mass fingerprinting and peptide fragment analysis
• robust high-capacity ion trap nanoESI/MSn for peptide fragment analysis, detailed characterization and quantitation

Data Interpretation:

    * powerful BioTools software for data interpretation

Knowledge Generation:

• ProteinScape database system for proteomics project management

Workflow Control

For the fast identification of large numbers of proteins from 2D gels, the intelligent use of available MS technologies is crucial: Use the fast MALDI-TOF and TOF/TOF technologies for the first rounds of screening, and reduce the samples numbers to a manageable size for in-depth LC-MS/MS analysis!

The workflow can be adjusted to analyze a maximum of samples, or to analyze fewer samples with maximal depth – even with MSn! This is managed by PROTEINEER’s search result-driven workflow control WARP™ (Workflow Administration by Result-driven Processing).

Depending on the search result from a protein mass fingerprint (PMF), TOF/TOF acquisitions for a sample on the same MALDI target will only be triggered in cases where identification failed – and automated LC-MS/MS will only be triggered if the upstream analyses failed. This dynamic sample-specific strategy provides outstanding success rates of protein identification at a minimum of analysis time.

Technical Specifications:

• Integrated mass-spec based system solution for protein identification and characterization from 2D gels
• Completely based on the MTP format, with transponder-coded sample tracking
• Powerful Daltonics mass spectrometers and robotics
• Proprietary break-through AnchopChip technology: 100 fmol protein-in-gel sensitivity, no further sample purification needed, about 70% of the sample amount left for ESI-MS/MS experiments
• Efficient bioinformatics tools for in-depth data interpretation
• Comprehensive database solution for bulky project data
• WARP: integrated MALDI/ESI-strategy with search result-driven workflow control
• Know-how from the expert labs inside: Robotics and database jointly developed with PROT@GEN
• Modular, scalable system – customizable to individual and increasing demands.
• PROTEINEER is a contribution to the HUPO Human Brain Proteome Project

For research use only. Not for use for diagnostic purposes.

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